Comparative analysis of the polymerase chainreaction and the hybridcapture assay for the detection of human papilomavírus infection

Authors

  • Maria Odete O. Carvalho Universidade Federal Fluminense
  • Fernanda N. Carestiato Universidade Federal Fluminense
  • Marisa O. Ribeiro
  • Marcia Marinho
  • Fabio M. Barbosa
  • Licínio E. Silva Universidade Federal Fluminense
  • Trude Dimetz
  • Ledy H.S. Oliveira Universidade Federal Fluminense
  • Silvia M.B. Cavalcanti Universidade Federal Fluminense

Keywords:

HPV, SIL, cancer, hybrid capture, PCR

Abstract

Introduction: cervical cancer is the second major cause of death from cancer in female worldwide. From 2000, World Health Organization acceptedthe Human Papillomavirus (HPV) as the etiological agents of cervical cancer. Nevertheless, the diagnosis of such viral infections still needs improve-ments. Objective:to evaluate the use of molecular methodologies to access HPV DNA, in association to Cytopathology, the classical method ofscreening cervical lesions. Method: the research was conducted by using samples from cervical smears of women attended at Laboratórios Dr SérgioFranco, at Rio de Janeiro, among 2000. They were analyzed by the hybrid capture assay II (HCA II) and the polymerase chain reaction (PCR) withgeneric primers MY 09/MY 11. The study was composed by 40 patients aging from 17 to 41 years old. The comparative analysis between HCA II andPCR was conducted with the objective to determine the sensibility and the specificity of HCA II. Results: HPV was detected in 75% of the samples, byHCA II, while PCR showed HPV in 70% of the patients. Cytopathology was used as a reference method of screening the lesions. The three testsshowed absolute agreement when cytopathology referred the sample as NORMAL, HPV and High grade lesion (HSIL). Disagreements occurred whencytopathology pointed low squamous intraepithelial lesions (LSIL), in which HCA II showed 100% of HPV detection and PCR pointed only 85% ofDNA detection. After comparing the two molecular techniques with cytopathology results, we observed that in HCA II (p = 0) as well as in PCR (p =0.002) a positive association was demonstrated. Conclusion: hence, we concluded that, despite a few disagreements, HCA II showed to be sensitiveand specific enough to be used in combination with cytopathology. Besides that, the evaluation of viral load measures obtained by HCA II showed tobe related to the severity of the lesion and merits further studies to analyze possible association to risk of progression to malignancy.

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Author Biographies

Maria Odete O. Carvalho, Universidade Federal Fluminense

Aluna do Curso de Mestrado em Patologia Experimental, bolsista CAPES.

Fernanda N. Carestiato, Universidade Federal Fluminense

Aluna do Curso de Mestrado em Patologia Experimental, bolsista CAPES.

Marisa O. Ribeiro

Gerente técnica do Laboratório Sérgio Franco. 

Marcia Marinho

Diretora do Laboratório Sérgio Franco.

Fabio M. Barbosa

Responsável pelo Setor de Informática do Laboratório Sérgio Franco.

Licínio E. Silva, Universidade Federal Fluminense

Prof. Adjunto do Departamento de Estatística, Especialista em Matemática Aplicada a Sistemas, Universidade Federal Fluminense.

Trude Dimetz

Consultora Científica do Laboratório Sérgio Franco.

Ledy H.S. Oliveira, Universidade Federal Fluminense

Prof. Adjunta do Departamento de Microbiologia e Parasitologia.

Silvia M.B. Cavalcanti, Universidade Federal Fluminense

Prof. Adjunta do Departamento de Microbiologia e Parasitologia.

Published

2004-02-27

How to Cite

1.
Carvalho MOO, Carestiato FN, Ribeiro MO, Marinho M, Barbosa FM, Silva LE, et al. Comparative analysis of the polymerase chainreaction and the hybridcapture assay for the detection of human papilomavírus infection. DST [Internet]. 2004 Feb. 27 [cited 2024 Nov. 22];16(1):26-30. Available from: https://bdst.emnuvens.com.br/revista/article/view/497

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Section

Original Article